GK060 ScienCell 人類晝夜節(jié)律qPCR檢測試劑盒,Human Circadian Rhythm qPCR Array Kit

ScienCell 研究實驗室（ScienCell）是一家生物技術(shù)公司，其使命是實驗和治療用細(xì)胞和細(xì)胞相關(guān)產(chǎn)品的研究和發(fā)展。 ScienCell為研究界提供各種高質(zhì)量的正常的人類和動物細(xì)胞，細(xì)胞培養(yǎng)基和試劑，培養(yǎng)基添加因子，細(xì)胞來源的RNA，cDNA和蛋白質(zhì)。ScienCell的科學(xué)家們以改善生活質(zhì)量，實現(xiàn)細(xì)胞的能力，打擊人類退化性疾病為目的研究和開發(fā)各種細(xì)胞治療策略。ScienCell總部設(shè)在美國加利福尼亞州圣地亞哥，公司成立于1999年。

北京諾博萊德作為ScienCell品牌的經(jīng)銷商，為客戶提供，正規(guī)進(jìn)口，貨期穩(wěn)定，折扣好的服務(wù)標(biāo)準(zhǔn)。

GK060 ScienCell人類晝夜節(jié)律qPCR檢測試劑盒，Human Circadian Rhythm qPCR Array Kit，產(chǎn)品簡介：

產(chǎn)品品牌: ScienCell

產(chǎn)品貨號：GK060

產(chǎn)品名稱：GK060 ScienCell人類晝夜節(jié)律qPCR檢測試劑盒，Human Circadian Rhythm qPCR Array Kit

產(chǎn)品規(guī)格：One 96-well plate, one 20ul reaction per well

GK060 ScienCell人類晝夜節(jié)律qPCR檢測試劑盒，Human Circadian Rhythm qPCR Array Kit， 產(chǎn)品說明：

ScienCell的GeneQuery™人類晝夜節(jié)律qPCR陣列試劑盒（GQH-CIR）分析了88個關(guān)鍵基因，這些基因參與了大約24小時周期內(nèi)發(fā)生的調(diào)節(jié)變化。 晝夜節(jié)律主要是對光變化的反應(yīng)，存在于大多數(shù)生物體中。 不要與生物鐘混淆，晝夜節(jié)律會影響睡眠模式、激素釋放、體溫和其他穩(wěn)態(tài)功能。 以下是如何根據(jù)其功能對所包含的基因進(jìn)行分組的簡要示例：

時鐘調(diào)節(jié)： ARNTL， BHLHE40， CLOCK， PER1， RORA

酪蛋白激酶：CSNK1A1、CSNK1D、CSNK1E、CSNK2A1

CREB途徑：CAMK2A，HTR7，MAPK1，PRKACB，AANAT

轉(zhuǎn)錄因子：SMAD4，IRF1，NFIL3，RORC，SP1，

GeneQuery™ qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒，每個孔包含一個引物組，可識別并有效擴(kuò)增特定靶基因的 cDNA。精心設(shè)計的引物確保：（i）qPCR分析中的優(yōu)良退火溫度為65°C（含2 mM Mg2+且不含DMSO）;（ii）除非另有說明，否則引物集可識別靶基因的所有已知轉(zhuǎn)錄本變體;（iii）只有一個基因被擴(kuò)增。每個引物組都已通過qPCR和熔解曲線分析和凝膠電泳進(jìn)行了驗證。

ScienCell's GeneQuery™ Human Circadian Rhythm qPCR Array Kit (GQH-CIR) profiles 88 key genes involved in the regulating changes that occur on a roughly 24-hr cycle.  Circadian rhythms are primarily responses to changes in light and are found in most living organisms.  Not to be confused with biological clocks, circadian rhythms can influence sleep patterns, hormone release, body temperature, and other homeostatic functions.  Below are brief examples of how included genes may be grouped according to their function:

·         Clock regulation: ARNTL, BHLHE40, CLOCK, PER1, RORA

·         Casein kinases: CSNK1A1, CSNK1D, CSNK1E, CSNK2A1

·         CREB pathway: CAMK2A, HTR7, MAPK1, PRKACB, AANAT

·         Transcription factors: SMAD4, IRF1, NFIL3, RORC, SP1,

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg²⁺and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.

GeneQuery™ 人類晝夜節(jié)律 qPCR 陣列板布局*（8 個粗體和斜體對照）

提示：基因選擇可能會根據(jù)新的研究和開發(fā)進(jìn)行更新

ScienCell基因表達(dá)分析產(chǎn)品訂購信息：

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